Zegarra-Moran O, Moran O
Settore di Biofisica, Scuola Internazionale Superiore di Studi Avanzati, Italy.
Exp Brain Res. 1993;95(1):65-9. doi: 10.1007/BF00229654.
Voltage-dependent calcium channels of cerebellar granule cells maintained in a Ca(2+)-free depolarising solution were recorded using the cell-attached configuration of the patch-clamp technique. An increase in the maximum open probability of calcium channels and a shift in their activation curve toward more hyperpolarizing potentials were found in the presence of glutamate, a natural, excitatory amino acid. Such an increase in the activity of calcium channels was not due to ionic fluxes activated by glutamate, and was probably produced by a second messenger pathway triggered by the binding of glutamate to its receptor.
采用膜片钳技术的细胞贴附式记录方式，记录了在无钙去极化溶液中维持的小脑颗粒细胞的电压依赖性钙通道。在天然兴奋性氨基酸谷氨酸存在的情况下，发现钙通道的最大开放概率增加，且其激活曲线向更超极化的电位偏移。钙通道活性的这种增加并非由谷氨酸激活的离子通量所致，可能是由谷氨酸与其受体结合触发的第二信使途径产生的。
