Lachumanan R, Devi S, Cheong Y M, Rodda S J, Pang T
Institute of Advanced Studies, University of Malaya, Kuala Lumpur.
Infect Immun. 1993 Oct;61(10):4527-31. doi: 10.1128/iai.61.10.4527-4531.1993.
Binding studies of 160 overlapping, synthetic octapeptides from the hydrophilic regions of the Sta58 major outer membrane protein of Rickettsia tsutsugamushi with sera from patients with scrub typhus revealed 15 immunodominant peptides which are recognized by all the sera tested. Further analysis of the specificity of peptide binding with five of these peptides indicated that the peptides showed significantly stronger binding to scrub typhus patients' sera than they did to sera from patients with other febrile illnesses common in the region, i.e., malaria, dengue fever, typhoid fever, and leptospirosis. The main antibody class binding to these peptides appears to be immunoglobulin M, and there appears to be little correlation between reactivity with peptides and antibody titers measured by the indirect immunoperoxidase test.
用恙虫病东方体Sta58主要外膜蛋白亲水区域的160个重叠合成八肽与恙虫病患者血清进行结合研究，发现了15个免疫显性肽，所有检测血清均可识别这些肽。对其中5个肽的肽结合特异性进行进一步分析表明，这些肽与恙虫病患者血清的结合明显强于与该地区其他常见发热性疾病患者血清的结合，即疟疾、登革热、伤寒和钩端螺旋体病。与这些肽结合的主要抗体类别似乎是免疫球蛋白M，肽反应性与间接免疫过氧化物酶试验测得的抗体滴度之间似乎几乎没有相关性。
