无需凝胶纯化的10(20)倍适体文库扩增。 - Suppr

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10(20)-fold aptamer library amplification without gel purification.

作者信息

Crameri A, Stemmer W P

机构信息

Affymax Research Institute, Palo Alto, CA 94304.

出版信息

Nucleic Acids Res. 1993 Sep 11;21(18):4410. doi: 10.1093/nar/21.18.4410.

DOI:10.1093/nar/21.18.4410

PMID:7692403

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC310094/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6535/310094/9a3b7336db5c/nar00067-0264-a.jpg

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本文引用的文献

Two binding modes in Escherichia coli single strand binding protein-single stranded DNA complexes. Modulation by NaCl concentration.大肠杆菌单链结合蛋白-单链DNA复合物中的两种结合模式。受氯化钠浓度的调节。

J Biol Chem. 1985 Mar 25;260(6):3594-603.

Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.指数富集的配体系统进化：噬菌体T4 DNA聚合酶的RNA配体

Science. 1990 Aug 3;249(4968):505-10. doi: 10.1126/science.2200121.

Selection of single-stranded DNA molecules that bind and inhibit human thrombin.结合并抑制人凝血酶的单链DNA分子的筛选。

Nature. 1992 Feb 6;355(6360):564-6. doi: 10.1038/355564a0.

In vitro selection of RNA molecules that bind specific ligands.结合特定配体的RNA分子的体外筛选。

Nature. 1990 Aug 30;346(6287):818-22. doi: 10.1038/346818a0.

Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures.体外筛选可折叠成特定配体结合结构的单链DNA分子。

Nature. 1992 Feb 27;355(6363):850-2. doi: 10.1038/355850a0.

RNA pseudoknots that inhibit human immunodeficiency virus type 1 reverse transcriptase.

Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):6988-92. doi: 10.1073/pnas.89.15.6988.

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