Deldar A, Stevens C E
In Vitro Hematopoiesis Laboratory, Lilly Research Laboratories, Eli Lilly and Company, Greenfield, Indiana 46140.
Toxicol Pathol. 1993;21(2):231-40. doi: 10.1177/019262339302100216.
In vitro models of hematopoiesis are used increasingly in investigative hematopathology. Such models complement in vivo animal testing and have been shown to be predictive for hematotoxicity associated with anticancer and antiviral agents in humans. In vitro models of hematopoiesis consist of short-term cloning assays for various hematopoietic progenitor cells and long-term functional assays for the marrow hematopoietic microenvironment. In our laboratories, the cloning assays have been used as investigative tools to study the pathogenetic mechanisms of drug-induced blood disorders and as screening systems to investigate the possible hematotoxic potential of candidate drugs in various animal species. Data in support of these applications are presented in this paper.
造血的体外模型在血液病理学研究中的应用日益广泛。此类模型可补充体内动物试验，并且已证明能够预测人类中与抗癌和抗病毒药物相关的血液毒性。造血的体外模型包括针对各种造血祖细胞的短期克隆测定以及针对骨髓造血微环境的长期功能测定。在我们实验室中，克隆测定已被用作研究药物诱导的血液疾病发病机制的研究工具，以及作为研究各种动物物种中候选药物可能的血液毒性潜力的筛选系统。本文展示了支持这些应用的数据。
