Huang J, Aoyama K, Ueda A
Department of Environmental Medicine, Faculty of Medicine, Kagoshima University, Japan.
Arch Toxicol. 1993;67(6):373-8. doi: 10.1007/BF01977397.
Groups of guinea pigs were exposed via inhalation to toluene diisocyanate (TDI) ranging from 0.02 to 1.0 ppm for 3 h/day on 5 consecutive days. Three weeks later, guinea pigs were challenged with TDI-GSA conjugates. Evaluations were based on TDI specific antibodies, pulmonary response and antigen stimulated histamine release from lung mast cells (LMC). The results indicated that both the antibody titer and the severity of pulmonary response followed a concentration-dependent pattern, with corresponding threshold levels. Antibodies and pulmonary responses were undetectable in animals exposed to 0.02 ppm TDI; however, exposure to 0.2 ppm TDI resulted in significant antibody production and pulmonary responses. Animals showed higher antibody titers and more severe pulmonary responses as TDI concentration increased from 0.6 ppm to 1.0 ppm. A discrepancy which was noted between antibody production and pulmonary responses was thought to be relevant to the discrimination of the potentials of antigen stimulated histamine release from LMC mediating between these two responses.
将豚鼠分组，连续5天每天吸入浓度范围为0.02至1.0 ppm的甲苯二异氰酸酯（TDI），每天3小时。三周后，用TDI - GSA缀合物对豚鼠进行激发试验。评估基于TDI特异性抗体、肺部反应以及抗原刺激的肺肥大细胞（LMC）组胺释放。结果表明，抗体滴度和肺部反应的严重程度均呈浓度依赖性模式，并具有相应的阈值水平。暴露于0.02 ppm TDI的动物中未检测到抗体和肺部反应；然而，暴露于0.2 ppm TDI会导致显著的抗体产生和肺部反应。随着TDI浓度从0.6 ppm增加到1.0 ppm，动物表现出更高的抗体滴度和更严重的肺部反应。抗体产生与肺部反应之间存在的差异被认为与区分LMC介导的这两种反应之间抗原刺激的组胺释放潜能有关。
