Alam R, Lewis D M, Olenchock S A
Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, W.Va.
J Allergy Clin Immunol. 1988 Mar;81(3):598-604.
Previous studies have demonstrated that grain dust can stimulate lymphocyte proliferation and the production of interleukin-1 by macrophages. This study was undertaken to investigate whether grain dust could stimulate the production of histamine-releasing factor (HRF) by guinea pig spleen cells. We also studied the direct action of grain dust on guinea pig lung mast cells and basophils. Plastic nonadherent cells from immunized animals were cultured for 24 hours in the presence of grain dust or lipopolysaccharide, and the cell-free supernatants were assayed for HRF activity in the mast cell and basophil histamine release test. Lung mast cells were isolated by enzymatic digestion and discontinuous Percoll gradient centrifugation. It has been demonstrated that grain dust stimulated the production of HRF by spleen cells from the immunized animals but not from the control animals. Stimulation of spleen cells with lipopolysaccharide did not enhance the HRF production. Gel chromatography of grain dust-stimulated supernatant revealed that HRF has a molecular weight in the range of 50 to 70 kd and 5 to 8 kd. We also found that grain dust directly released significant amount of histamine from both mast cells and basophils. The results of this study suggest that grain dust contains some potent active substances that can activate lymphocytes, mast cells, and basophils.
以往的研究表明，谷物粉尘可刺激淋巴细胞增殖以及巨噬细胞产生白细胞介素-1。本研究旨在调查谷物粉尘是否能刺激豚鼠脾细胞产生组胺释放因子（HRF）。我们还研究了谷物粉尘对豚鼠肺肥大细胞和嗜碱性粒细胞的直接作用。将来自免疫动物的塑料非贴壁细胞在谷物粉尘或脂多糖存在的情况下培养24小时，然后在肥大细胞和嗜碱性粒细胞组胺释放试验中检测无细胞上清液的HRF活性。通过酶消化和不连续 Percoll 梯度离心分离肺肥大细胞。结果表明，谷物粉尘刺激了免疫动物脾细胞产生HRF，但未刺激对照动物的脾细胞。用脂多糖刺激脾细胞并未增强HRF的产生。对谷物粉尘刺激的上清液进行凝胶色谱分析显示，HRF的分子量在50至70kd和5至8kd范围内。我们还发现，谷物粉尘可直接使肥大细胞和嗜碱性粒细胞释放大量组胺。本研究结果表明，谷物粉尘含有一些能激活淋巴细胞、肥大细胞和嗜碱性粒细胞的强效活性物质。
