Dietrich C U, Jacobsen B B, Starklint H, Heim S
Department of Medical Genetics, Odense University, Denmark.
Genes Chromosomes Cancer. 1993 Aug;7(4):240-4. doi: 10.1002/gcc.2870070410.
An embryonal rhabdomyosarcoma was analyzed cytogenetically. In primary cultures fed a serum-containing medium, 11 clones with karyotypic abnormalities were found. One had trisomy 8 only. The other 10 clones had trisomy 8 as well as additional evolutionary changes that included trisomy for part or all of chromosome 2, isochromosomes for the short and long arms of chromosome 11, isochromosomes for the long arm of chromosome 8, and extra copies of chromosome 8, some of which had an interstitial deletion in 8q. In those primary cultures that had grown in a chemically defined, serum-free medium and in all passaged cultures, trisomy 8 was the only aberration. Our findings and a survey of published information point to gain of one chromosome 8 as a frequent primary karyotypic abnormality in embryonal rhabdomyosarcomas. Trisomy for part or all of chromosomes 2 and 11 and additional gains of chromosome 8 material seem to be common secondary changes.
对一例胚胎性横纹肌肉瘤进行了细胞遗传学分析。在含有血清的培养基中进行原代培养时，发现了11个具有核型异常的克隆。其中一个仅为8号染色体三体。其他10个克隆除了8号染色体三体外，还存在其他进一步的染色体变化，包括2号染色体部分或全部三体、11号染色体短臂和长臂的等臂染色体、8号染色体长臂的等臂染色体以及8号染色体的额外拷贝，其中一些在8q有中间缺失。在化学限定的无血清培养基中生长的原代培养物以及所有传代培养物中，8号染色体三体是唯一的异常。我们的研究结果以及对已发表信息的调查表明，获得一条8号染色体是胚胎性横纹肌肉瘤常见的原发性核型异常。2号和11号染色体部分或全部三体以及8号染色体物质的额外增加似乎是常见的继发性变化。
