Deguchi T, Doi T, Ehara H, Ito S, Takahashi Y, Nishino Y, Fujihiro S, Kawamura T, Komeda H, Horie M
Department of Urology, Gifu University School of Medicine, Tsukasa-Machi, Japan.
Cancer Res. 1993 Nov 15;53(22):5350-4.
We have developed a highly sensitive method for detecting prostate cancer cells using reverse transcriptase-polymerase chain reaction (RT-PCR) with primers specific for prostate-specific antigen gene. Forty-four lymph nodes obtained from 22 patients with prostate cancers were analyzed by RT-PCR to detect metastatic prostate cancer cells. RT-PCR could detect prostate-specific antigen mRNA in five lymph nodes with histologically and/or immunohistochemically identifiable metastases and in four lymph nodes with negative histological and immunohistochemical analyses for metastases. RT-PCR was a more sensitive method than histology and immunohistochemistry in detecting metastatic prostate cancer cells and could be applied for diagnosing micrometastases of prostate cancer to lymph nodes. This highly sensitive RT-PCR will be a relevant tool to allow a more accurate clinical assessment of lymph node metastases of prostate cancer and to understand lymphatic dissemination of prostate cancer biologically.
我们开发了一种高度灵敏的方法，利用逆转录酶-聚合酶链反应（RT-PCR）及前列腺特异性抗原基因特异性引物来检测前列腺癌细胞。对22例前列腺癌患者获取的44个淋巴结进行RT-PCR分析，以检测转移性前列腺癌细胞。RT-PCR能够在5个经组织学和/或免疫组织化学鉴定有转移的淋巴结以及4个组织学和免疫组织化学分析转移阴性的淋巴结中检测到前列腺特异性抗原mRNA。在检测转移性前列腺癌细胞方面，RT-PCR是比组织学和免疫组织化学更灵敏的方法，可用于诊断前列腺癌向淋巴结的微转移。这种高度灵敏的RT-PCR将成为一种相关工具，有助于对前列腺癌淋巴结转移进行更准确的临床评估，并从生物学角度了解前列腺癌的淋巴扩散情况。
