Deguchi T, Yang M, Nishino Y, Ehara H, Takahashi Y, Kawata Y, Ito S, Ito Y, Doi T
Department of Urology, Gifu University School of Medicine.
Hinyokika Kiyo. 1996 Oct;42(10):787-90.
We detected micrometastatic prostate cancer cells in lymph nodes and bone marrow using reverse transcriptase-polymerase chain reaction (RT-PCT) specific for prostate-specific antigen (PSA). RT-PCR revealed PSA mRNA in two lymph nodes obtained from two patients with negative histological and immunohistochemical analyses for lymph node metastases. Of 26 patients with negative bone scan imaging, 7 had PSA mRNA detected in the bone marrow by RT-PCR. The RT-PCR will be a relevant tool to allow a more accurate clinical assessment of lymph node and bone metastases in patients with prostate cancer.
我们使用针对前列腺特异性抗原（PSA）的逆转录聚合酶链反应（RT-PCR）在淋巴结和骨髓中检测到了微转移前列腺癌细胞。RT-PCR在两名组织学和免疫组化分析淋巴结转移均为阴性的患者所获取的两个淋巴结中发现了PSA mRNA。在26例骨扫描成像为阴性的患者中，有7例通过RT-PCR在骨髓中检测到了PSA mRNA。RT-PCR将成为一种有助于对前列腺癌患者的淋巴结和骨转移进行更准确临床评估的相关工具。
