Heinrich J C, Tabler M, Louis C
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Crete, Greece.
Dev Genet. 1993;14(4):258-65. doi: 10.1002/dvg.1020140403.
We have transformed Drosophila melanogaster with a DNA construct encoding a catalytic antisense RNA directed against the primary white gene RNA transcript. Total RNA isolated from transformed flies was shown to catalyze the specific cleavage of in vitro transcribed substrate RNA, indicating the expression of a functional ribozyme. Moreover, transgenic lines carrying homozygous copies of this construct depict a further clear-cut reduction in eye pigmentation when present in a genetic background that has a priori reduced levels of white gene expression.
我们用一种DNA构建体转化了黑腹果蝇，该构建体编码一种针对主要白色基因RNA转录本的催化性反义RNA。从转化后的果蝇中分离出的总RNA被证明能催化体外转录的底物RNA的特异性切割，这表明功能性核酶的表达。此外，携带该构建体纯合拷贝的转基因品系，当存在于白色基因表达水平先天降低的遗传背景中时，眼睛色素沉着会进一步明显减少。
