Orfila C, Bompart G, Lepert J C, Suc J M, Girolami J P
INSERM CJF 91-07, CHU Rangueil, Toulouse, France.
Histochem J. 1993 Sep;25(9):664-9. doi: 10.1007/BF00157880.
The renal origin of kallikrein is now clearly established. However, the presence of kallikrein in urine raises questions about a possible physiological role of this enzyme at the urinary level. We have already demonstrated the presence of kallikrein-like substance in rat ureter. For establishing the continuity of the presence of kallikrein-like substance along the urinary tract we have studied the localization of immunoreactive kallikrein-like substance in urinary bladder of the normal rat by immunohistochemical methods for light- and electron-microscopy, using an antibody against rat urinary kallikrein. By light microscopy, kallikrein-like substance was found to be associated with the lamina propria, which is the connective tissue component which constitutes one layer of the bladder wall. Weak staining was present in the smooth-muscle layer. By immuno-electron microscopy, kallikrein-like substance was localized in fibroblasts which were present in the connective tissue and which penetrated into the layer of smooth muscle; immunoreactivity was observed in endoplasmic reticulum, Golgi apparatus and free polyribosomes. Immunolabelling was demonstrated in no other part of the wall bladder and in no other cellular component. The continuity of the presence of kallikrein-like substance from the kidney to the urinary bladder gives new indications concerning the significance of this system in renal physiology.
激肽释放酶的肾脏起源现已明确确立。然而，尿液中激肽释放酶的存在引发了关于该酶在尿路水平可能的生理作用的疑问。我们已经证明大鼠输尿管中存在激肽释放酶样物质。为了确定激肽释放酶样物质在尿路中的连续性，我们使用抗大鼠尿激肽释放酶抗体，通过光镜和电镜免疫组织化学方法研究了正常大鼠膀胱中免疫反应性激肽释放酶样物质的定位。通过光镜观察，发现激肽释放酶样物质与固有层相关，固有层是构成膀胱壁一层的结缔组织成分。平滑肌层有弱阳性染色。通过免疫电镜观察，激肽释放酶样物质定位于结缔组织中的成纤维细胞，这些成纤维细胞深入平滑肌层；在内质网、高尔基体和游离多核糖体中观察到免疫反应性。在膀胱壁的其他部位和其他细胞成分中均未显示免疫标记。激肽释放酶样物质从肾脏到膀胱的连续性为该系统在肾脏生理学中的意义提供了新的线索。
