Orstavvik T B, Inagami T
J Histochem Cytochem. 1982 Apr;30(4):385-90. doi: 10.1177/30.4.7037945.
The anatomical relationship between kallikrein and renin in the rat kidney was investigated immunohistochemically by the peroxidase-antiperoxidase method. Kallikrein was localized to the convoluted distal tubule, starting at a point, distal to the juxtaglomerular apparatus, where the thick ascending limb of loop of Henle transformed into the convoluted distal tubule. The thick ascending limb was identified by its content of uromucoid (Tamm-Horsfall glycoprotein). Kallikrein was never observed within the juxtaglomerular apparatus itself. The kallikrein-containing tubule ended where the distal tubule submerged into the collecting duct. Renin was found in epitheloid cells of the afferent arteriole. When neighboring sections were stained for kallikrein and renin, respectively, no close anatomical relationship was observed between the kallikrein-containing and the renin-containing structures.
采用过氧化物酶-抗过氧化物酶法，通过免疫组织化学方法研究了大鼠肾脏中激肽释放酶与肾素之间的解剖学关系。激肽释放酶定位于远曲小管，起始于近球小体远端的一点，即髓袢升支粗段转变为远曲小管的部位。髓袢升支粗段通过其尿粘蛋白（Tamm-Horsfall糖蛋白）的含量得以识别。在近球小体本身内从未观察到激肽释放酶。含有激肽释放酶的小管在远曲小管汇入集合管处终止。肾素存在于入球小动脉的上皮样细胞中。当相邻切片分别用激肽释放酶和肾素染色时，未观察到含激肽释放酶结构与含肾素结构之间存在紧密的解剖学关系。
