Valenta R, Ferreira F, Grote M, Swoboda I, Vrtala S, Duchêne M, Deviller P, Meagher R B, McKinney E, Heberle-Bors E
Institute of General and Experimental Pathology, University of Vienna, Austria.
J Biol Chem. 1993 Oct 25;268(30):22777-81.
Profilin is a low molecular weight protein involved in the organization of the mammalian and protozoan cytoskeleton as well as in signal transduction. In this study, profilin is identified as an actin-binding protein in higher plants which is present in monocot and dicot angiosperms. Birch pollen profilin and actin can be copurified as a complex, and purified recombinant birch profilin can be used as an affinity matrix to obtain birch pollen actin. The binding of 125I-labeled recombinant birch pollen profilin to plant and animal actins can be blocked by profilin-specific antibodies that react with different epitopes of birch profilin. One of the blocking antibodies was raised against the 25 COOH-terminal amino acids indicating the importance of this region in the profilactin complex formation.
肌动蛋白结合蛋白是一种低分子量蛋白质，参与哺乳动物和原生动物细胞骨架的组织以及信号转导。在本研究中，肌动蛋白结合蛋白被鉴定为高等植物中的一种肌动蛋白结合蛋白，存在于单子叶和双子叶被子植物中。桦树花粉肌动蛋白结合蛋白和肌动蛋白可以作为复合物共纯化，纯化的重组桦树肌动蛋白结合蛋白可以用作亲和基质来获得桦树花粉肌动蛋白。125I标记的重组桦树花粉肌动蛋白结合蛋白与植物和动物肌动蛋白的结合可以被与桦树肌动蛋白结合蛋白不同表位反应的肌动蛋白结合蛋白特异性抗体阻断。其中一种阻断抗体是针对25个COOH末端氨基酸产生的，表明该区域在肌动蛋白结合蛋白复合物形成中的重要性。
