Wiley E L, Milchgrub S, Freeman R G, Kim E S
Department of Pathology, University of Texas, Southwestern Medical Center at Dallas 75235-9072.
J Cutan Pathol. 1993 Aug;20(4):337-43. doi: 10.1111/j.1600-0560.1993.tb01272.x.
Thirty-one dermal appendage tumors of sweat gland differentiation including 7 spiradenomas (SPA), 8 cylindromas (CYL), 8 acrospiromas (ACS), and 8 chondroid syringomas (CS) were analyzed using antibodies to epithelial membrane antigen (EMA), cytokeratin (AE1, AE3, CAM 5.2, 34BE12), S-100 protein, actin (ACT), and desmin (DES) to characterize the immunocytochemical profile of benign sweat gland tumors. Cytokeratin expression was variable; AE1, 34BE12, AE3, and CAM 5.2 were present in 31, 24, 23, and 22 tumors respectively; 29 tumors contained EMA. Seventeen tumors, (6 SPA, 8 CYL, 2 ACS, 1 CS) stained with antibody to alpha smooth muscle actin, and 26 (7 SPA, 7 CYL, 4 ACS, 8 CS) expressed S-100 protein. Although some prior studies had reported actin filaments on electron microscopy in both spiradenoma and cylindroma, these tumors have previously been considered to be negative for myoepithelial differentiation. All spiradenomas and cylindromas we studied demonstrated actin and/or S-100 protein positivity in basal epithelial cells, consistent with myoepithelial differentiation. The organization of actin and S-100 protein positivity displayed by the spiradenomas and cylindromas we studied suggests that the tumors are differentiated towards the secretory portion of the eccrine sweat gland.
对31例汗腺分化的皮肤附属器肿瘤进行了分析，其中包括7例螺旋腺瘤（SPA）、8例圆柱瘤（CYL）、8例顶泌螺旋腺瘤（ACS）和8例软骨样汗管瘤（CS），使用抗上皮膜抗原（EMA）、细胞角蛋白（AE1、AE3、CAM 5.2、34BE12）、S-100蛋白、肌动蛋白（ACT）和结蛋白（DES）的抗体来表征良性汗腺肿瘤的免疫细胞化学特征。细胞角蛋白表达存在差异；AE1、34BE12、AE3和CAM 5.2分别在31、24、23和22个肿瘤中存在；29个肿瘤含有EMA。17个肿瘤（6例SPA、8例CYL、2例ACS、1例CS）用抗α平滑肌肌动蛋白抗体染色，26个（7例SPA、7例CYL、4例ACS、8例CS）表达S-100蛋白。尽管一些先前的研究在电子显微镜下报道了螺旋腺瘤和圆柱瘤中均有肌动蛋白丝，但这些肿瘤以前被认为肌上皮分化呈阴性。我们研究的所有螺旋腺瘤和圆柱瘤在基底上皮细胞中均显示肌动蛋白和/或S-100蛋白阳性，这与肌上皮分化一致。我们研究的螺旋腺瘤和圆柱瘤所显示的肌动蛋白和S-100蛋白阳性的组织方式表明，这些肿瘤向小汗腺的分泌部分分化。
