Patriarca C, Roncalli M, Gambacorta M, Cominotti M, Coggi G, Viale G
II Department of Pathology, University of Milan, School of Medicine, Italy.
J Pathol. 1993 Sep;171(1):5-11. doi: 10.1002/path.1711710104.
Thirty cases of hepatocellular carcinoma (HCC) were investigated immunocytochemically for expression of beta 1 integrin molecule and of collagen IV. Immunoreactivity was related to the tumour proliferation index, as detected by PCNA immunostaining, and to tumour size and grade. Membrane beta 1 integrin immunoreactivity was detected in the neoplastic cells of all cases, though two different staining patterns were clearly recognized. In 14 cases, beta 1 integrin immunoreactivity was confined to the cell-stroma interface, showing the same polarized pattern as the non-neoplastic cell counterpart. This staining pattern was associated significantly (P < 0.0001) with low PCNA labelling (i.e. less than 20 per cent of neoplastic cells showing nuclear immunostaining. Conversely, 16 cases showed non-polarized pericellular beta 1 integrin immunostaining. This staining pattern was significantly associated (P < 0.0001) with high PCNA labelling (more than 20 per cent of immunoreactive cells) and with tumour size greater than 4 cm in diameter (P < 0.0001). beta 1 Integrin, collagen IV, and PCNA immunoreactivities, however, did not correlate with the histological grade. The data emphasize that neoplastic progression of HCCs may be correlated with an aberrant expression of adhesion molecules and with a disruption of the collagen IV complement of basal membranes.
对30例肝细胞癌（HCC）进行免疫细胞化学研究，以检测β1整合素分子和IV型胶原的表达。免疫反应性与通过PCNA免疫染色检测到的肿瘤增殖指数、肿瘤大小和分级相关。在所有病例的肿瘤细胞中均检测到膜β1整合素免疫反应性，不过可清楚识别出两种不同的染色模式。在14例病例中，β1整合素免疫反应性局限于细胞-基质界面，呈现出与非肿瘤细胞对应物相同的极化模式。这种染色模式与低PCNA标记显著相关（P<0.0001）（即核免疫染色的肿瘤细胞少于20%）。相反，16例病例显示细胞周围β1整合素免疫染色呈非极化状态。这种染色模式与高PCNA标记（免疫反应性细胞超过20%）以及直径大于4 cm的肿瘤大小显著相关（P<0.0001）。然而，β1整合素、IV型胶原和PCNA免疫反应性与组织学分级无关。这些数据强调，HCC的肿瘤进展可能与黏附分子的异常表达以及基底膜IV型胶原成分的破坏相关。
