Harrison R F, Reynolds G M, Rowlands D C
Department of Pathology, Medical School, University of Birmingham, U.K.
J Pathol. 1993 Oct;171(2):115-22. doi: 10.1002/path.1711710208.
The possibility that proliferating cell nuclear antigen (PCNA) is expressed by non-proliferating liver cells was investigated. Liver biopsies from 107 patients were investigated, which included histologically normal liver, metastatic tumour, and inflammatory lesions. PCNA was detected using immunohistochemical staining with the monoclonal antibody PC10. This was compared with the proportion of proliferating cells as assessed by immunostaining for the Ki-67 antigen using the monoclonal antibody MIB 1. Most cases of histologically normal liver showed few PC10-positive cells. PCNA-positive hepatocytes far outnumbered those positive with MIB 1 in specimens showing metastatic tumour or an inflammatory cell infiltrate. There was no relation between the degree of PCNA overexpression and the type of tumour present or the nature of the inflammatory lesion. Other cell types, including the biliary epithelium, did not show this large difference between the proportions of PC10- and MIB 1-positive cells. It is concluded that non-proliferating hepatocytes increase their levels of PCNA in a wide variety of pathological conditions. This may be mediated by cytokines released by tumour cells or inflammatory cells.
对非增殖性肝细胞表达增殖细胞核抗原（PCNA）的可能性进行了研究。对107例患者的肝活检组织进行了研究，其中包括组织学正常的肝脏、转移性肿瘤和炎性病变。使用单克隆抗体PC10通过免疫组织化学染色检测PCNA。将其与使用单克隆抗体MIB 1通过Ki-67抗原免疫染色评估的增殖细胞比例进行比较。大多数组织学正常的肝脏病例显示PC10阳性细胞很少。在显示转移性肿瘤或炎性细胞浸润的标本中，PCNA阳性肝细胞的数量远远超过MIB 1阳性的肝细胞。PCNA过表达程度与存在的肿瘤类型或炎性病变的性质之间没有关系。包括胆管上皮在内的其他细胞类型，在PC10和MIB 1阳性细胞比例之间没有显示出这种巨大差异。得出的结论是，在多种病理情况下，非增殖性肝细胞会增加其PCNA水平。这可能由肿瘤细胞或炎性细胞释放的细胞因子介导。
