铜绿假单胞菌杀白细胞素对兔多形核白细胞化学发光反应的启动效应。

Priming effect of pseudomonal leukocidin on chemiluminescence response of rabbit polymorphonuclear leukocytes.

作者信息

Nishiya H, Kunii O, Noda M

机构信息

Second Department of Internal Medicine, University of Teikyo, Tokyo, Japan.

出版信息

Microbiol Immunol. 1993;37(7):531-6. doi: 10.1111/j.1348-0421.1993.tb01673.x.

DOI:10.1111/j.1348-0421.1993.tb01673.x

PMID:7694054

Abstract

To clarify effects of pseudomonal leukocidin (42.5 kd) on chemiluminescence (CL) production of polymorphonuclear leukocytes (PMNs), rabbit PMNs were stimulated by zymosan or phorbol myristate acetate (PMA) after pretreatment with the leukocidin, which by itself stimulated little chemiluminescence response. The extent of CL responses stimulated by zymosan or PMA was respectively 5.3- or 3.5-fold greater in leukocidin (1.5 micrograms/ml)-pretreated PMNs than in non-pretreated ones. The priming effect of the leukocidin was greater than that of G-CSF and related to some steps before NADPH oxidase activation. The increased CL productions might be related to tissue damages caused by pseudomonal infections in vivo.

摘要

为阐明铜绿假单胞菌杀白细胞素（42.5千道尔顿）对多形核白细胞（PMN）化学发光（CL）产生的影响，在用几乎不刺激化学发光反应的杀白细胞素预处理后，用酵母聚糖或佛波醇肉豆蔻酸酯乙酸盐（PMA）刺激兔PMN。在杀白细胞素（1.5微克/毫升）预处理的PMN中，由酵母聚糖或PMA刺激的CL反应程度分别比未预处理的PMN高5.3倍或3.5倍。杀白细胞素的启动作用大于粒细胞集落刺激因子（G-CSF），且与NADPH氧化酶激活之前的某些步骤有关。CL产生增加可能与体内铜绿假单胞菌感染引起的组织损伤有关。