Ruitenberg E J, Buys J, Meeuwsen F
Zentralbl Bakteriol Orig A. 1975 Dec;233(4):561-4.
A description is given of a mechanized micro complement-fixation test for the serodiagnosis of human toxoplasmosis. Besides a reduction in the quantity of reagents by using a microplate a considerable advantage of the test is the mechanization of actually all steps. Examination of large numbers of sera can be realized in a relatively short period of time. In order to test the sensitivity of the micro CF test comparative studies were carried out with the macro CF test and the immunofluorescence (IF) test on 549 human sera. The micro CF test proved to be more sensitive than the macro CF test. The results obtained with the micro CF test were in better agreement with the IF test than those obtained with the macro CF test.
本文描述了一种用于人类弓形虫病血清诊断的机械化微量补体结合试验。除了使用微孔板减少试剂用量外，该试验的一个显著优点是几乎所有步骤都实现了机械化。可以在相对较短的时间内对大量血清进行检测。为了测试微量补体结合试验的敏感性，对549份人类血清进行了微量补体结合试验与常量补体结合试验及免疫荧光试验的对比研究。结果表明，微量补体结合试验比常量补体结合试验更敏感。与常量补体结合试验相比，微量补体结合试验的结果与免疫荧光试验的结果更一致。
