Grabovskaia K B, Golubkov V I
Zh Mikrobiol Epidemiol Immunobiol. 1975 Nov(11):45-9.
The authors present materials for comparison of three methods of E. coli infection by the RNA of the MS2 bacteriophage. The cells accepted the RNA at the stage of spheroplasts (obtained with the aid of a lysozyme and EDTA) with the efficacy of up to 1.5 X 10(5) IC/mug of the RNA. Diethylpyrocarbonate was used as a transfection stimulant. The product of transfection was identical to the MS2 bacteriophage. The efficacy of transfection in the calcinated system and on the frozen-defrosted bacteria was lower than on the spheroplasts (10(2) IC/mug of the RNA) which was largely associated with the formation of "latent infectious centres". Formation of latent negative colonies was characteristic of bacteriophage MS2, but formation of these colonies was intensified when calcinated cells were used for transfection.
作者展示了用于比较通过MS2噬菌体RNA感染大肠杆菌的三种方法的材料。细胞在原生质球阶段（借助溶菌酶和乙二胺四乙酸获得）接受RNA，其效率高达每微克RNA 1.5×10⁵感染性单位（IC）。焦碳酸二乙酯用作转染刺激剂。转染产物与MS2噬菌体相同。在煅烧系统中和冷冻解冻细菌上的转染效率低于在原生质球上的转染效率（每微克RNA 10² IC），这在很大程度上与“潜伏感染中心”的形成有关。潜伏阴性菌落的形成是噬菌体MS2的特征，但当使用煅烧细胞进行转染时，这些菌落的形成会加剧。
