el-Salhy M, Stenling R, Grimelius L
Dept. of Internal Medicine, University Hospital, Umeå, Sweden.
Scand J Gastroenterol. 1993 Sep;28(9):809-15. doi: 10.3109/00365529309104014.
Histologically normal liver biopsy specimens from patients with Hodgkin's lymphoma were investigated with three immunohistochemical methods for the occurrence of peptidergic nerve fibers and endocrine cells. Numerous immunoreactive nerve fibers were seen with antisera against peripheral nerves markers (neuron-specific enolase, neurofilament protein, and S-100). These nerve fibers were localized in the tunica media of branches of both the hepatic artery and portal vein, around the bile ducts, and in the connective tissue of the interlobular septa. In the liver, 10 types of peptidergic nerve fibers were detected: glucagon-, glucagon-like peptide- (GLP), somatostatin-, neuropeptide Y- (NPY), vasoactive intestinal polypeptide-, neurotensin-, gastrin/cholecystokinin C-terminus-, substance P-, serotonin-, and galanin-immunoreactive nerve fibers. GLP-, somatostatin-, NPY-, neurotensin-, substance P-, and galanin-immunoreactive nerve fibers were abundant; the other nerve fibers were scarce. The nerve fibers showed two distinct patterns of distribution: they occurred in the blood vessel wall and in connective tissue of the interlobular septum. Pancreatic polypeptide- and NPY-immunoreactive cells were found among the lining epithelial cells of the bile ducts in the interlobular septum.
采用三种免疫组织化学方法对霍奇金淋巴瘤患者组织学正常的肝活检标本进行研究，以检测肽能神经纤维和内分泌细胞的存在情况。使用抗周围神经标志物（神经元特异性烯醇化酶、神经丝蛋白和S-100）的抗血清可观察到大量免疫反应性神经纤维。这些神经纤维位于肝动脉和门静脉分支的中膜、胆管周围以及小叶间隔的结缔组织中。在肝脏中，检测到10种肽能神经纤维：胰高血糖素、胰高血糖素样肽（GLP）、生长抑素、神经肽Y（NPY）、血管活性肠肽、神经降压素、胃泌素/胆囊收缩素C端、P物质、5-羟色胺和甘丙肽免疫反应性神经纤维。GLP、生长抑素、NPY、神经降压素、P物质和甘丙肽免疫反应性神经纤维数量丰富；其他神经纤维较少。神经纤维呈现两种不同的分布模式：它们存在于血管壁和小叶间隔的结缔组织中。在小叶间隔胆管的内衬上皮细胞中发现了胰多肽和NPY免疫反应性细胞。
