Kameda Y
Department of Anatomy, Kitasato University School of Medicine, Kanagawa, Japan.
Cell Tissue Res. 1999 Dec;298(3):437-47. doi: 10.1007/s004419900121.
The chicken carotid body receives numerous branches from the vagus nerve, especially distal (nodose) ganglion, and the recurrent laryngeal nerve. Dense networks of peptidergic nerve fibers immunoreactive for substance P, calcitonin gene-related peptide (CGRP), galanin, vasoactive intestinal peptide (VIP) and neuropeptide Y are distributed in and around the carotid body. Substance-P- and CGRP-immunoreactive fibers projecting to the chicken carotid body mainly come from the vagal ganglia. In the present study, various types of denervation experiments were performed in order to clarify the origins of VIP-, galanin- and neuropeptide-Y-immunoreactive fibers in the chicken carotid bodies. After nodose ganglionectomy, midcervical vagotomy or excision of the recurrent laryngeal nerve, VIP-, galanin- and neuropeptide-Y-immunoreactive fibers were unchanged in the carotid body region. Furthermore, these peptidergic fibers remained unaffected even by removal of the nodose ganglion in conjunction with severance of the recurrent laryngeal nerve that induced a marked decrease in TuJ1-immunoreactive fibers in the carotid body region. VIP-, galanin- and neuropeptide-Y-immunoreactive fibers are densely distributed around the arteries supplying the carotid body in normal chickens. The peptidergic fibers around the arteries were also unaffected after the denervation experiments. However, after removal of the 14th cervical ganglion of the sympathetic trunk, which lies close to the vertebral artery on the root of the brachial plexus and issues prominent branches to the artery, VIP-, galanin- and neuropeptide-Y-immunoreactive fibers almost disappeared in the carotid body region. The ganglion contained many VIP-, galanin- and neuropeptide-Y-immunoreactive neurons. Thus it is clear that VIP-, galanin- and neuropeptide-Y-immunoreactive fibers in the chicken carotid body region are mainly derived from the 14th cervical sympathetic ganglion via the vertebral artery.
鸡的颈动脉体接受来自迷走神经的众多分支，尤其是来自远端（结状）神经节和喉返神经的分支。对P物质、降钙素基因相关肽（CGRP）、甘丙肽、血管活性肠肽（VIP）和神经肽Y呈免疫反应的肽能神经纤维密集网络分布于颈动脉体及其周围。投射至鸡颈动脉体的P物质和CGRP免疫反应性纤维主要来自迷走神经节。在本研究中，进行了各种类型的去神经实验，以阐明鸡颈动脉体中VIP、甘丙肽和神经肽Y免疫反应性纤维的起源。在进行结状神经节切除术、颈中部迷走神经切断术或喉返神经切除术后，颈动脉体区域内的VIP、甘丙肽和神经肽Y免疫反应性纤维没有变化。此外，即使同时切除结状神经节并切断喉返神经，导致颈动脉体区域内TuJ1免疫反应性纤维显著减少，这些肽能纤维仍未受影响。在正常鸡中，VIP、甘丙肽和神经肽Y免疫反应性纤维密集分布于供应颈动脉体的动脉周围。去神经实验后，动脉周围的肽能纤维也未受影响。然而，在切除位于臂丛神经根处靠近椎动脉并向该动脉发出明显分支的第14颈交感神经节后，颈动脉体区域内的VIP、甘丙肽和神经肽Y免疫反应性纤维几乎消失。该神经节含有许多VIP、甘丙肽和神经肽Y免疫反应性神经元。因此，很明显，鸡颈动脉体区域内的VIP、甘丙肽和神经肽Y免疫反应性纤维主要通过椎动脉来自第14颈交感神经节。
