在相应的微分干涉差（诺马斯基）图像中，牛蛙（Rana catesbeiana）颈动脉迷路中FMRF酰胺免疫反应性神经纤维的分布。
Distribution of FMRFamide-immunoreactive nerve fibers in the carotid labyrinth of the bullfrog, Rana catesbeiana in corresponding differential interference-contrast (Nomarski) images.
作者信息
Kusakabe T, Kawakami T, Takenaka T
机构信息
Department of Anatomy, Yokohama City University School of Medicine, Japan.
出版信息
Arch Histol Cytol. 1993 Aug;56(3):277-82. doi: 10.1679/aohc.56.277.
Immunoreactivities for FMRFamide and substance P (SP) in the carotid labyrinth of the bullfrog were detected using the peroxidase-antiperoxidase method, and the results compared with corresponding differential interference-contrast (Nomarski) images. Colocalization of both peptides was determined by the indirect double immunofluorescence method. Immunoreactivities for FMRFamide and SP were found in nerve fibers distributed in the intervascular stroma of the labyrinth. The FMRFamide-immunoreactive fibers were less numerous than the SP-immunoreactive fibers. In the Nomarski image, FMRFamide-fibers were recognized in relief, with most of them located near the walls of blood vessels. All FMRFamide-fibers coexisted with SP. The results suggest that FMRFamide-immunoreactive fibers are also involved in local vascular regulation of the carotid labyrinth.
采用过氧化物酶抗过氧化物酶法检测牛蛙颈动脉迷路中FMRF酰胺和P物质（SP）的免疫反应性，并将结果与相应的微分干涉相差（诺马斯基）图像进行比较。通过间接双重免疫荧光法确定两种肽的共定位。在迷路血管间基质中分布的神经纤维中发现了FMRF酰胺和SP的免疫反应性。FMRF酰胺免疫反应性纤维比SP免疫反应性纤维数量少。在诺马斯基图像中，FMRF酰胺纤维呈浮雕状可见，其中大部分位于血管壁附近。所有FMRF酰胺纤维都与SP共存。结果表明，FMRF酰胺免疫反应性纤维也参与颈动脉迷路的局部血管调节。
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