Kusakabe T, Kawakami T, Takenaka T
Department of Anatomy, Yokohama City University School of Medicine, Japan.
Cell Tissue Res. 1994 Oct;278(1):41-4. doi: 10.1007/BF00305776.
Indirect double immunofluorescence labelling for demonstrating nine neuropeptides in the kidney of the bullfrog, Rana catesbeiana, revealed for the first time the occurrence, distribution, and coexistence of certain neuropeptides in the kidney of the submammalian vertebrates. Substance P, neuropeptide Y, and calcitonin gene-related peptide were localized in nerve fibers distributed along the afferent arterioles connected with the glomeruli, and along the capillary network between uriniferous tubules. Neuropeptide Y and calcitonin gene-related peptide immunoreactive fibers were more numerous than substance P immunoreactive fibers. In these two regions, about one half of the neuropeptide Y or calcitonin gene-related peptide fibers contained substance P. No immunoreactivity of vasoactive intestinal polypeptide, somatostatin, FMRFamide, or leucine- and methionine-enkephalins was detected in the bullfrog kidney.
通过间接双重免疫荧光标记法来显示牛蛙（Rana catesbeiana）肾脏中的九种神经肽，首次揭示了某些神经肽在非哺乳类脊椎动物肾脏中的存在、分布及共存情况。P物质、神经肽Y和降钙素基因相关肽定位于沿与肾小球相连的入球小动脉以及沿肾小管间毛细血管网分布的神经纤维中。神经肽Y和降钙素基因相关肽免疫反应性纤维比P物质免疫反应性纤维数量更多。在这两个区域，约一半的神经肽Y或降钙素基因相关肽纤维含有P物质。在牛蛙肾脏中未检测到血管活性肠肽、生长抑素、FMRF酰胺或亮氨酸脑啡肽和甲硫氨酸脑啡肽的免疫反应性。
