Tomimoto H, Akiguchi I, Wakita H, Kimura J, Hori K, Yodoi J
Department of Neurology, Faculty of Medicine, Kyoto University, Japan.
Brain Res. 1993 Oct 15;625(1):1-8. doi: 10.1016/0006-8993(93)90130-f.
Distribution of adult T cell leukemia derived factor (ADF/TRX), a human thioredoxin homologue, was studied by immunohistochemistry in gerbil brain during reperfusion after transient cerebral ischemia. In control brains, immunoreactivity was observed widely in the central nervous system, including the ependyma, tanycytes, endothelial cells as well as subcommisural organs, and weakly in the neuronal cell bodies. During reperfusion, ADF/TRX was expressed in glial cells in the CA1 and dentate hilus of the hippocampus, and in a few cases in the lateral portion of the caudateputamen. ADF/TRX positive glias first appeared after reperfusion for 24 h, and the intensity of staining peaked at 72 h and diminished after 7 days of reperfusion. They were concentrated around microvessels and identified to be astroglia by double labeling immunohistochemistry with glial fibrillary acidic protein as marker. Immunoblotting analysis demonstrated an increase of ADF/TRX in the postischemic hippocampus. Astroglial expression of ADF/TRX in postischemic injuries suggests a role in neuroprotection by hydrogen peroxide reducing and protein-refolding activities or in modulation of local immune responses.
采用免疫组织化学方法，研究了成年T细胞白血病衍生因子（ADF/TRX，一种人类硫氧还蛋白同源物）在沙土鼠短暂性脑缺血再灌注期间脑内的分布情况。在对照脑中，免疫反应广泛见于中枢神经系统，包括室管膜、伸长细胞、内皮细胞以及连合下器官，在神经元细胞体中则较弱。在再灌注期间，ADF/TRX在海马体CA1区和齿状回门的胶质细胞中表达，少数情况下在尾状壳核外侧部分表达。ADF/TRX阳性胶质细胞在再灌注24小时后首次出现，染色强度在72小时达到峰值，再灌注7天后减弱。它们集中在微血管周围，通过以胶质纤维酸性蛋白为标记物的双重免疫组织化学鉴定为星形胶质细胞。免疫印迹分析表明，缺血后海马体中ADF/TRX增加。缺血后损伤中星形胶质细胞表达ADF/TRX提示其在通过过氧化氢还原和蛋白质重折叠活性进行神经保护或调节局部免疫反应中发挥作用。
