Miyazawa T, Sato K
Department of Neurosurgery, National Defense Medical College, Tokorozawa, Japan.
Brain Dev. 1994 Nov-Dec;16(6):432-6. doi: 10.1016/0387-7604(94)90002-7.
The fluorescence intensity (FI) on immunohistochemical staining with a monoclonal antibody (Mab 171 B5) against a synaptic vesicle-associated protein (SVP-38) was measured in the CA1 sector of the hippocampus of brains obtained from hydrocephalic and non-hydrocephalic HTX-rats aged 1, 7, 14, 21 and 28 days. FI was significantly increased on the 21st and 28th postnatal days in hydrocephalic rats as compared to in non-hydrocephalic rats, despite the presence of marked morphological stretching and rotation of the dendritic arborization of Golgi-impregnated neurons in the CA1 sector. The increase in FI appeared to indicate an increase in synaptic density. As previous reports have indicated a marked reduction in SVP-38 FI in the thin cerebral cortex of 28th postnatal day hydrocephalic brains, our findings may suggest that hippocampal synaptogenesis is more resilient than that of the cerebral cortex in hydrocephalic brains.
用抗突触小泡相关蛋白（SVP - 38）的单克隆抗体（Mab 171 B5）进行免疫组织化学染色，测量1、7、14、21和28日龄脑积水和非脑积水HTX大鼠大脑海马CA1区的荧光强度（FI）。与非脑积水大鼠相比，脑积水大鼠在出生后第21天和第28天FI显著增加，尽管CA1区高尔基染色神经元的树突分支存在明显的形态学拉伸和旋转。FI的增加似乎表明突触密度增加。正如之前的报道指出，出生后第28天脑积水大脑的薄皮层中SVP - 38 FI显著降低，我们的研究结果可能表明，在脑积水大脑中，海马的突触发生比大脑皮层更具弹性。
