Hutchings D L, Wilson S H
Agriculture and Agri-Food Canada, Animal Diseases Research Institute, Nepean, Ontario.
Am J Vet Res. 1995 Jan;56(1):27-33.
Lymphocyte stimulation tests (LST), performed using 6 antigen preparations, were compared individually and in pairs. The tests were performed on 433 blood samples collected from elk in Mycobacterium bovis-infected herds. These elk were killed as part of Agriculture and Agri-Food Canada's bovine tuberculosis eradication policy, and mycobacterial culture results were obtained from tissues of each animal. The LST, which had the highest total sum of sensitivity and specificity, was a comparative test that used M bovis purified protein derivative (PPD) and M paratuberculosis (johnin) PPD. This test had a sensitivity of 76%, with confidence limits (CL) of 63 to 85% for this estimate, and specificity of 77% (CL, 72 to 81%). The LST, using only M bovis PPD antigen, had a sensitivity of 70% (CL, 57 to 80%) and specificity of 74% (CL, 69 to 79%); when it was compared with culture results, using the kappa statistic, agreement was only 32%. This indicated that the LST identified different elk than did M bovis isolation tests.
使用6种抗原制剂进行的淋巴细胞刺激试验（LST）被单独及成对比较。这些试验在从感染牛分枝杆菌的鹿群采集的433份血样上进行。这些鹿作为加拿大农业及农业食品部牛结核病根除政策的一部分被宰杀，并从每只动物的组织中获得了分枝杆菌培养结果。敏感性和特异性总和最高的LST是一种使用牛分枝杆菌纯化蛋白衍生物（PPD）和副结核分枝杆菌（副结核菌素）PPD的比较试验。该试验的敏感性为76%，此估计值的置信区间（CL）为63%至85%，特异性为77%（CL，72%至81%）。仅使用牛分枝杆菌PPD抗原的LST敏感性为70%（CL，57%至80%），特异性为74%（CL，69%至79%）；当与培养结果比较时，使用kappa统计量，一致性仅为32%。这表明LST识别出的鹿与牛分枝杆菌分离试验识别出的不同。
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