Surujballi Om, Lutze-Wallace Cyril, Turcotte Claude, Savic Mirjana, Stevenson Dan, Romanowska Anna, Monagle Wendy, Berlie-Surujballi Gloria, Tangorra Erin
Canadian Food Inspection Agency, Mycobacterial Diseases Centre of Expertise, Ottawa Laboratory Fallowfield, Ottawa, Ontario, Canada.
Can J Vet Res. 2009 Jul;73(3):161-6.
After histopathological examination of a lesion found in a herd member returned a diagnosis of mycobacteriosis, a farmed herd (n = 47) of elk (Cervus elaphus nelsoni) and red deer (C. elaphus elaphus) was investigated for bovine tuberculosis with a battery of antemortem and postmortem diagnostic tests. Every animal was tested with the mid-cervical tuberculin skin test; all 47 had negative results. All of the 16 adult animals and 15 of the 31 calves (approximately 2-years-old) were blood-tested with a lymphocyte stimulation test (LST) and a fluorescence polarization assay (FPA), which detects antibody to the MPB70 protein antigen. At necropsy of the 31 blood-tested animals, tissues were harvested for histopathological examination and culture of mycobacteria. Mycobacterium bovis was isolated from 16 of the 31 animals, and a scotochromogen was also isolated from 1 of the 16 whose tissues yielded M. bovis. Each of these 16 animals, 15 of which were calves, also received a histopathological diagnosis of mycobacteriosis. Other species of mycobacteria, including those belonging to the M. avium and M. terrae complexes, were isolated from an additional 7 animals. The FPA was scored "positive" or "suspect" for 16 animals, 13 (81%) of which were culture-positive for M. bovis. The other 3 animals that were culture-positive for M. bovis had negative FPA results. Of the 3 FPA-positive or FPA-suspect animals that were culture-negative, 2 were suspected to have mycobacteriosis on the basis of the histopathological examination. The 7 animals from which Mycobacterium species other than M. bovis were cultured were all FPA-negative. The only animal with positive LST results was also FPA-positive and culture-positive for M. bovis. The M. bovis isolates had an identical spoligotype pattern, with an octal code of 664073777777600. This is the first report of the isolation and identification of this strain type in Canada.
在对一群鹿群中发现的病变进行组织病理学检查后，诊断为分枝杆菌病，随后对一群圈养的驼鹿（Cervus elaphus nelsoni）和马鹿（C. elaphus elaphus）（n = 47）进行了一系列生前和死后诊断测试，以检测牛结核病。每只动物都进行了颈中部结核菌素皮肤试验；所有47只动物的结果均为阴性。16只成年动物和31只犊牛（约2岁）中的15只进行了淋巴细胞刺激试验（LST）和荧光偏振测定法（FPA）血液检测，后者可检测针对MPB70蛋白抗原的抗体。在对31只进行血液检测的动物进行尸检时，采集组织进行组织病理学检查和分枝杆菌培养。从31只动物中的16只分离出牛分枝杆菌，在16只组织中分离出牛分枝杆菌的动物中，有1只还分离出了暗产色分枝杆菌。这16只动物中的每一只，其中15只是犊牛，也都得到了分枝杆菌病的组织病理学诊断。从另外7只动物中分离出了其他分枝杆菌物种，包括属于鸟分枝杆菌和地分枝杆菌复合体的那些。FPA对16只动物的评分是“阳性”或“可疑”，其中13只（81%）牛分枝杆菌培养呈阳性。其他3只牛分枝杆菌培养呈阳性的动物FPA结果为阴性。在3只FPA阳性或FPA可疑但培养阴性的动物中，有2只根据组织病理学检查怀疑患有分枝杆菌病。从其培养出非牛分枝杆菌物种的7只动物FPA均为阴性。唯一LST结果呈阳性的动物FPA也呈阳性，且牛分枝杆菌培养呈阳性。牛分枝杆菌分离株具有相同的间隔寡核苷酸分型模式八进制编码为664073777777600。这是在加拿大首次报告分离和鉴定出这种菌株类型。
