Thoen C O, Quinn W J, Miller L D, Stackhouse L L, Newcomb B F, Ferrell J M
College of Veterinary Medicine, Iowa State University, Ames 50011.
J Vet Diagn Invest. 1992 Oct;4(4):423-7. doi: 10.1177/104063879200400410.
A naturally occurring outbreak of Mycobacterium bovis infection in captive wild elk (wapiti) in Montana was confirmed by mycobacteriologic examination. Twenty-eight of 143 elk responded to M. bovis purified protein derivative (PPD) tuberculin injected intradermally in the cervical region (SCT). The results of comparative cervical tuberculin skin tests conducted within 9 days of SCT revealed greater responses to M. bovis PPD tuberculin than to M. avium PPD tuberculin in 23 of 28 elk responding. At necropsy, several grossly visible tuberculous lesions were observed in the parenchyma of the lung, thoracic lymph nodes, and submandibular lymph nodes. Microscopic examination of appropriately stained tissue sections revealed the presence of granulomatous lesions containing acid-fast bacilli. An enzyme-linked immunosorbent assay (ELISA) was developed using a sarkosyl extract of M. bovis (antigen) and peroxidase-labeled protein G (conjugate); reactions were detected in the sera of 8 of 9 elk responding to M. bovis PPD tuberculin. Lymphocyte blastogenic assay responses were detected using M. bovis antigens in 7 of 9 elk positive on skin tests using M. bovis PPD.
通过细菌学检查确认了蒙大拿州圈养野生麋鹿（马鹿）中自然发生的牛分枝杆菌感染疫情。143头麋鹿中有28头对颈部皮内注射的牛分枝杆菌纯蛋白衍生物（PPD）结核菌素（SCT）有反应。在SCT后9天内进行的对比颈部结核菌素皮肤试验结果显示，在28头有反应的麋鹿中，有23头对牛分枝杆菌PPD结核菌素的反应比对鸟分枝杆菌PPD结核菌素的反应更强。尸检时，在肺实质、胸淋巴结和下颌下淋巴结中观察到几个肉眼可见的结核病变。对适当染色的组织切片进行显微镜检查，发现存在含有抗酸杆菌的肉芽肿病变。使用牛分枝杆菌的 Sarkosyl 提取物（抗原）和过氧化物酶标记的蛋白G（结合物）开发了一种酶联免疫吸附测定（ELISA）；在9头对牛分枝杆菌PPD结核菌素有反应的麋鹿中，有8头的血清检测到反应。在9头使用牛分枝杆菌PPD进行皮肤试验呈阳性的麋鹿中，有7头使用牛分枝杆菌抗原检测到淋巴细胞增殖试验反应。
