The fibrous architecture of the rat periodontal ligament in cryosections examined by scanning electron microscopy.

Cryosections through the incisor and molar teeth of the rat mandible were examined, with and without hyaluronidase pretreatment, in the scanning electron microscope. In the fully erupted molar teeth the fibres of the periodontal ligament were organized into bundles which crossed the space from the alveolus to the cementum and inserted into the associated mineralized tissues. In the erupting incisor teeth, three distinct zones were evident. The outer alveolar and cemental zones were composed of coarse fibre bundles which inserted into the adjacent mineralized tissues, while the middle zone was composed of collagenous laminates running along the axis of the tooth. These observations confirm a proposed model for the structure of the erupting periodontal ligament and suggest that the method used will provide further information about the role of the ligament in tooth support and eruption.