Sloan P, Carter D H, Kielty C M, Shuttleworth C A
Department of Oral and Maxillofacial Medicine and Surgery, Turner Dental School, Manchester, UK.
Histochem J. 1993 Jul;25(7):523-30. doi: 10.1007/BF00159289.
The distribution of collagen types I and VI has been examined in the periodontal ligaments of rat incisor and molar teeth using cryosections and immunohistochemical staining procedures. The stain for collagen type I was uniform in all the ligaments examined. Uniform staining for collagen type VI was evident only in the ligament of the fully erupted molars, and the stain was absent from the mid-zone of the ligaments of the erupting molars and incisors. The staining pattern of the collagen type VI antibodies is consistent with previous reports which have suggested that the removal of collagen type VI precedes the degradation of major banded collagen fibrils in remodelling connective tissues. The removal of collagen type VI from the mid-zone of the incisor ligament may precede the remodelling events which facilitate tooth eruption.
利用冷冻切片和免疫组织化学染色程序，对大鼠切牙和磨牙的牙周韧带中I型和VI型胶原蛋白的分布进行了检测。在所检测的所有韧带中，I型胶原蛋白染色均匀。仅在完全萌出磨牙的韧带中，VI型胶原蛋白染色明显均匀，而在正在萌出的磨牙和切牙韧带的中间区域则无染色。VI型胶原蛋白抗体的染色模式与先前的报道一致，这些报道表明在重塑结缔组织中，VI型胶原蛋白的去除先于主要带状胶原纤维的降解。切牙韧带中间区域VI型胶原蛋白的去除可能先于促进牙齿萌出的重塑事件。
