Hamid M A, Iwaku M, Hoshino E
Department of Operative Dentistry, Niigata University School of Dentistry, Japan.
Arch Oral Biol. 1994 Nov;39(11):967-72. doi: 10.1016/0003-9969(94)90080-9.
Degradation of phenylalanine and leucine by resting cells of Eubacterium brachy ATCC 33089 were studied under strict anaerobic conditions. The effects of metronidazole and air on the metabolism were also studied. The organism principally produced phenylpropionate and isocaproate from phenylalanine and leucine, respectively. Other products were cinnamate and phenylacetate from phenylalanine, and alpha-ketoisocaproate and isovalerate from leucine. The organism also produced hydroxylated end-products, i.e. phenyllactate from phenylalanine and hydroxyisocaproate from leucine. When metronidazole was added to the reaction mixture, the production of phenylpropionate, cinnamate, phenylacetate, isocaproate, alpha-ketoisocaproate and isovalerate was inhibited, while that of hydroxylated products was not, suggesting that the organism has metronidazole-sensitive and -tolerant pathways of metabolism. A similar inhibitory effect was also found when the reaction was done aerobically, suggesting that the inhibitory mode of metronidazole is similar to that of oxygen.
在严格厌氧条件下，研究了短真杆菌ATCC 33089的静息细胞对苯丙氨酸和亮氨酸的降解作用。还研究了甲硝唑和空气对代谢的影响。该微生物主要分别从苯丙氨酸和亮氨酸产生苯丙酸酯和异己酸酯。其他产物是来自苯丙氨酸的肉桂酸酯和苯乙酸，以及来自亮氨酸的α-酮异己酸酯和异戊酸酯。该微生物还产生羟基化终产物，即来自苯丙氨酸的苯乳酸和来自亮氨酸的羟基异己酸。当向反应混合物中加入甲硝唑时，苯丙酸酯、肉桂酸酯、苯乙酸、异己酸酯、α-酮异己酸酯和异戊酸的产生受到抑制，而羟基化产物的产生不受抑制，这表明该微生物具有对甲硝唑敏感和耐受的代谢途径。当反应在有氧条件下进行时，也发现了类似的抑制作用，这表明甲硝唑的抑制模式与氧气的抑制模式相似。
