Sharma V, Knobloch T J, Benjamin D
Division of Hematology and Oncology, Arthur G. James Cancer Hospital and Research Institute, Ohio State University, Columbus 43210.
Biochem Biophys Res Commun. 1995 Mar 17;208(2):704-13. doi: 10.1006/bbrc.1995.1395.
Cytokine responses are dramatically affected when HIV-1 infected cells are activated with certain antigenic stimuli. We report the effects of HIV-1 tat gene in cytokine modulation, using HIV-1 tat transfected T (Jurkat) and B (Raji) cell lines. Studying the effect of tat and/or PMA + PHA on mRNA expression of 14 cytokines (IL-1 alpha, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, TNF-alpha, TNF-beta, GM-CSF, TGF-beta, IFN-gamma and MIP-1 alpha) illustrated differential effects. In addition to the varied effects of tat on the steady state levels of cytokine mRNAs, tat induced the secretion of TNF-beta preferentially in both B and T cell lines, either by itself as in Raji B cell line or synergistically upon PMA + PHA stimulation as in Jurkat T cell line.
当用某些抗原刺激激活HIV-1感染的细胞时，细胞因子反应会受到显著影响。我们使用HIV-1 tat转染的T（Jurkat）和B（Raji）细胞系，报告了HIV-1 tat基因在细胞因子调节中的作用。研究tat和/或PMA + PHA对14种细胞因子（IL-1α、IL-2、IL-4、IL-5、IL-6、IL-8、IL-10、IL-12、TNF-α、TNF-β、GM-CSF、TGF-β、IFN-γ和MIP-1α）mRNA表达的影响，结果显示出不同的效应。除了tat对细胞因子mRNA稳态水平的多种影响外，tat在B细胞系和T细胞系中均优先诱导TNF-β的分泌，在Raji B细胞系中是单独诱导，而在Jurkat T细胞系中则是在PMA + PHA刺激下协同诱导。
