Hardy T A, Wu D, Roach P J
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis 46202-5122.
Biochem Biophys Res Commun. 1995 Mar 17;208(2):728-34. doi: 10.1006/bbrc.1995.1398.
A Saccharomyces cerevisiae gene was identified that would encode a protein similar to the mammalian protein kinase glycogen synthase kinase-3 (GSK-3) and the Drosophila Zeste-White3/Shaggy gene product. The open reading frame predicts a 375 amino acid polypeptide with a putative protein kinase domain that displays 70% and 39% identity, respectively, to two known yeast proteins, Mds1p and Mck1p. The new gene, designated MRK1 (Mds1p Related Kinase), is located on chromosome IV. Disruption of MRK1 was not lethal and did not elicit any alteration in glycogen accumulation. In addition, an mck1 mds1 mrk1 triple disruptant was viable.
已鉴定出一种酿酒酵母基因，该基因编码的蛋白质与哺乳动物蛋白激酶糖原合酶激酶-3（GSK-3）以及果蝇的Zeste-White3/Shaggy基因产物相似。开放阅读框预测该基因编码一个含有375个氨基酸的多肽，其具有一个假定的蛋白激酶结构域，该结构域与两种已知的酵母蛋白Mds1p和Mck1p分别具有70%和39%的同源性。这个新基因被命名为MRK1（Mds1p相关激酶），位于第四条染色体上。MRK1基因的缺失并不致命，也不会引起糖原积累的任何改变。此外，mck1 mds1 mrk1三重缺失突变体是可存活的。
