Samko O T, Kalugin A A, El'darov M A, Karpychev I V, Anikeĭcheva N V, Khoroshutina E B, Skriabin K G, Librik G I, Sokolov N N
Bioorg Khim. 1994 Dec;20(12):1327-33.
New site-specific endonucleases BciBI and BciBII have been detected in Bacillus circulans. The enzymes were purified by fractionation of cell-free extract with polyethylene imine and ammonium sulphate (40-80% of saturation) followed by chromatography on DEAE-sepharose, blue-sepharose and phosphocellulose. The endonucleases BciBI and BciBII were separated only at the final step of the purification--by chromatography on the phosphocellulose column. The yields of BciBI and BciBII were 600 and 10,000 U/g of cells. It was found that restriction endonucleases BciBI and BciBII are isoschizomers of ClaI and BstNI, respectively.
在环状芽孢杆菌中检测到了新的位点特异性内切酶BciBI和BciBII。通过用聚乙烯亚胺和硫酸铵（饱和度为40 - 80%）对无细胞提取物进行分级分离，随后在DEAE - 琼脂糖、蓝色琼脂糖和磷酸纤维素上进行色谱分离来纯化这些酶。内切酶BciBI和BciBII仅在纯化的最后一步——通过磷酸纤维素柱色谱分离才得以分开。BciBI和BciBII的产量分别为每克细胞600单位和10,000单位。发现限制性内切酶BciBI和BciBII分别是ClaI和BstNI的同裂酶。
