Torchut E, Bourdillon C, Laval J M
Laboratoire de Technologie Enzymatique, URA 1442 du CNRS, Université de Technologie de Compiègne, France.
Biosens Bioelectron. 1994;9(9-10):719-23. doi: 10.1016/0956-5663(94)80070-7.
These studies develop a methodology to form supported phospholipid bilayers at an electrode/solution interface that models biological membrane systems. Two kinds of electrode were used, a planar gold electrode and a microporous aluminium oxide electrode on which octadecanethiol or octadecyltrichlorosilane was self-assembled. The supported lipidic structures were produced by transfer of a phospholipid monolayer by the Langmuir-Blodgett technique or by direct fusion of phospholipid vesicles. Ubiquinone was introduced into the lipidic structures during their formation; electrochemical measurements demonstrated the mobility of ubiquinone along the plane of the bilayer. A membrane enzyme, pyruvate oxidase from E. coli, was successfully incorporated into this artificial bilayer and was found to be able to exchange electrons with ubiquinone present in the bilayer.
这些研究开发了一种在电极/溶液界面形成模拟生物膜系统的支撑磷脂双层的方法。使用了两种电极，一种是平面金电极，另一种是在其上自组装了十八烷硫醇或十八烷基三氯硅烷的微孔氧化铝电极。通过朗缪尔-布洛杰特技术转移磷脂单层或直接融合磷脂囊泡来制备支撑脂质结构。在脂质结构形成过程中引入了泛醌；电化学测量表明泛醌在双层平面内具有流动性。一种膜酶，来自大肠杆菌的丙酮酸氧化酶，成功地整合到这种人工双层中，并且发现它能够与双层中存在的泛醌交换电子。
