Griffon G, Marchal C, Merlin J L, Marchal S, Parache R M, Bey P
Laboratoire de Recherche en Oncologie, Centre Alexis Vautrin, Vandoeuvre-les-Nancy, France.
Eur J Cancer. 1995;31A(1):85-91. doi: 10.1016/0959-8049(94)00377-h.
The radioresponsiveness of immunologically characterised (KL1, antivimentin and OC125) human ovarian carcinoma cells, obtained from effusions or solid tumours, was assayed in vitro using the multicellular tumour spheroids (MTS) three-dimensional model. Great interspecimen variabilities were observed in MTS doubling time (1.0-8.5 days), as well as in the doses inducing a 50% decrease in the MTS individual volume (ID50) (0.56-9.15 Gy), or in the overall population MTS number (SCD50) (1.9-15.7 Gy) and the residual/initial MTS individual volume ratio after 2 Gy irradiation (RSV2) (10-88%). The doubling time, DNA-ploidy and S-phase fraction did not correlate with the ID50. Significant correlations were found between the new parameters defined (RSV2 and ID50) and the SCD50, a well-accepted local control parameter. These parameters demonstrated their usefulness for studying the radiosensitivity of MTS prepared from human ovarian tumour biopsies.
采用多细胞肿瘤球体（MTS）三维模型，对从积液或实体瘤中获取的、经免疫特征鉴定（KL1、抗波形蛋白和OC125）的人卵巢癌细胞的放射反应性进行了体外测定。在MTS倍增时间（1.0 - 8.5天）、诱导MTS个体体积减少50%的剂量（ID50）（0.56 - 9.15 Gy）、总体群体MTS数量（SCD50）（1.9 - 15.7 Gy）以及2 Gy照射后残留/初始MTS个体体积比（RSV2）（10 - 88%）方面，观察到样本间存在很大差异。倍增时间、DNA倍性和S期分数与ID50无相关性。在定义的新参数（RSV2和ID50）与SCD50（一个广泛接受的局部控制参数）之间发现了显著相关性。这些参数证明了它们在研究从人卵巢肿瘤活检样本制备的MTS放射敏感性方面的有用性。
