Griffon-Etienne G, Merlin J L, Marchal C
Laboratoire de Recherche en Oncologie, Centre Alexis Vautrin, Vandoeuvre-les-Nancy Cedex, France.
Anticancer Drugs. 1996 Aug;7(6):660-70. doi: 10.1097/00001813-199608000-00007.
The association of taxoid derivatives (paclitaxel and docetaxel) with irradiation was evaluated in ovarian carcinoma cell lines (A2780 and CAVEOC-2) using the multicellular tumor spheroids (MTS) tridimensional model and compared to the conventional bidimensional model. The radiosensitivity parameters were the surviving fraction at 2 Gy, and alpha calculated using the linear-quadratic model for monolayer culture, the residual/control volume ratios at 2 Gy (RSV2) and doses inducing 50% decrease in MTS number (SCD50) calculated for spheroids. In A2780 monolayer culture, the combination was synergistic for paclitaxel and additive for docetaxel. In spheroids, both compounds induced a decrease in RSV2 and SCD50 in the two cell lines, and their combination with radiation was additive. Therefore, the radiosensitizing effect of taxoid derivatives was not constant in ovarian cell lines. The different results achieved in monolayer culture and in spheroids may suggest higher drug incorporation and fixation through the multiple cell layers of the spheroids than in monolayers.
使用多细胞肿瘤球体（MTS）三维模型，在卵巢癌细胞系（A2780和CAVEOC - 2）中评估了紫杉烷衍生物（紫杉醇和多西他赛）与辐射的联合作用，并与传统的二维模型进行了比较。放射敏感性参数包括2 Gy时的存活分数，以及使用单层培养的线性二次模型计算的α，2 Gy时的残余/对照体积比（RSV2）和针对球体计算的导致MTS数量减少50%的剂量（SCD50）。在A2780单层培养中，紫杉醇的联合作用是协同的，多西他赛的联合作用是相加的。在球体中，两种化合物在两种细胞系中均诱导RSV2和SCD50降低，并且它们与辐射的联合作用是相加的。因此，紫杉烷衍生物的放射增敏作用在卵巢细胞系中并不恒定。在单层培养和球体中获得的不同结果可能表明，与单层相比，通过球体的多个细胞层药物掺入和固定更高。
