Kadosawa T, Nozaki K, Sasaki N, Takeuchi A
Veterinary Hospital, Faculty of Agriculture, University of Tokyo, Japan.
J Vet Med Sci. 1994 Dec;56(6):1167-9. doi: 10.1292/jvms.56.1167.
A new cell line has been established directly from a spontaneous osteosarcoma on the femur of a 1.5-year-old male mongrel dog and named POS. Doubling time of the cells was approximately 33 hr. Morphologically, spherical cells, fibroblast-like cells, large or small polygonal cells and multinucleated giant cells seemed to be the major component of this cell line. The transmission electron microscopic feature of most of the cells was abundant dilated rough endoplasmic reticulum. Alkaline phosphatase activity as one of the osteoblastic properties was high in this cell line. The tumor tissue produced by the inoculation of the cells into nude mice was histologically identical to the original osteosarcoma.
从一只1.5岁雄性杂种犬股骨上的自发性骨肉瘤中直接建立了一种新的细胞系，并命名为POS。细胞的倍增时间约为33小时。形态学上，球形细胞、成纤维细胞样细胞、大小不等的多边形细胞和多核巨细胞似乎是该细胞系的主要组成部分。大多数细胞的透射电子显微镜特征是丰富扩张的粗面内质网。作为成骨细胞特性之一的碱性磷酸酶活性在该细胞系中较高。将细胞接种到裸鼠体内产生的肿瘤组织在组织学上与原始骨肉瘤相同。
