Jaffar-Bandjee M C, Carrère J, Bally M, Guy-Crotte O, Galabert C
Laboratoire de Biochimie, CERM, Hôpital Renée Sabran, Giens, Hyères, France.
Eur J Clin Chem Clin Biochem. 1994 Dec;32(12):893-9. doi: 10.1515/cclm.1994.32.12.893.
Using immunoenzymometric assays, the production of elastase, alkaline protease and exotoxin A was determined in culture supernatants of 35 strains of Pseudomonas aeruginosa isolated from patients suffering from cystic fibrosis. The assays were simple, specific, sensitive and reproducible, and permitted the determination of low levels of exoproteins. A large strain variability of exoprotein production was found. Most of the strains secreted all three exoproteins, but six out of the 35 strains (17%) did not secrete at least one of the three (< 0.3 microgram/l). A significant correlation was observed between elastase and exotoxin A productions (r = 0.697, p < 0.001).
采用免疫酶测定法，对从囊性纤维化患者分离出的35株铜绿假单胞菌培养上清液中的弹性蛋白酶、碱性蛋白酶和外毒素A的产生情况进行了测定。这些测定方法简单、特异、灵敏且可重复，能够测定低水平的外蛋白。发现外蛋白产生存在很大的菌株变异性。大多数菌株分泌所有三种外蛋白，但35株中有6株（17%）至少不分泌这三种外蛋白中的一种（<0.3微克/升）。观察到弹性蛋白酶和外毒素A的产生之间存在显著相关性（r = 0.697，p < 0.001）。
