Jagger K S, Robinson D L, Franz M N, Warren R L
J Clin Microbiol. 1982 Jun;15(6):1054-8. doi: 10.1128/jcm.15.6.1054-1058.1982.
Enzyme-linked immunosorbent assays were developed to measure serum antibody specific for Pseudomonas elastase, alkaline protease, and exotoxin A. Antibody responses to each Pseudomonas antigen were measured in cystic fibrosis (CF) patients who were not colonized with Pseudomonas aeruginosa, in those who were colonized, in those who were chronically infected with this organism, and in control subjects. Antibody levels for each antigen in the colonized and infected CF patients were higher than levels in uncolonized CF patients or non-CF control subjects. The antibody responses to elastase were similar in patients of the colonized and infected groups. However, infected CF patients had significantly elevated levels of antibody to exotoxin A (P less than 0.01) and alkaline protease (P less than 0.05) when compared with patients simply colonized with P. aeruginosa. These findings confirm that Pseudomonas alkaline protease, elastase, and exotoxin A are produced by Pseudomonas strains which colonize and infect CF patients. As an adjunct to established procedures (X-ray, microbiological culture, etc.), the antitoxin and anti-protease enzyme-linked immunosorbent assays may be clinically useful tests for differentiating colonized CF patients from those who have more severe Pseudomonas pulmonary infections.
已开发出酶联免疫吸附测定法来检测血清中针对铜绿假单胞菌弹性蛋白酶、碱性蛋白酶和外毒素A的特异性抗体。在未被铜绿假单胞菌定植的囊性纤维化（CF）患者、已被定植的患者、长期感染该菌的患者以及对照受试者中，检测对每种铜绿假单胞菌抗原的抗体反应。已被定植和感染的CF患者中每种抗原的抗体水平高于未被定植的CF患者或非CF对照受试者中的水平。定植组和感染组患者对弹性蛋白酶的抗体反应相似。然而，与单纯被铜绿假单胞菌定植的患者相比，感染的CF患者对外毒素A（P<0.01）和碱性蛋白酶（P<0.05）的抗体水平显著升高。这些发现证实，定植和感染CF患者的铜绿假单胞菌菌株会产生碱性蛋白酶、弹性蛋白酶和外毒素A。作为既定程序（如X线、微生物培养等）的辅助手段，抗毒素和抗蛋白酶酶联免疫吸附测定法可能是临床上区分定植的CF患者与患有更严重铜绿假单胞菌肺部感染患者的有用检测方法。
