Mg2+和ATP对合成肌球蛋白丝影响的动态光散射研究
Dynamic light scattering study of the effect of Mg2+ and ATP on synthetic myosin filaments.
作者信息
Takayama S, Fujime S
机构信息
Graduate School of Integrated Science, Yokohama City University, Japan.
出版信息
Biophys J. 1995 Feb;68(2):609-18. doi: 10.1016/S0006-3495(95)80222-5.
The dynamic light scattering (DLS) method provides us with information about the apparent diffusion coefficient, Dapp, as well as the static scattering intensity, Is, of particles in solution. For long but thin rods with length L and diameter d, the dependence on L and d of Dapp is quite different from that of Is. By means of DLS we studied synthetic myosin filaments of rabbit skeletal muscle in solution at pH 8.3 and 10 degrees C. It appeared that Mg2+ ions induced thickening and lengthening of the filaments, whereas ATP (and ADP) induced thinning and shortening (depolymerization) of the filaments. When ATP was added to the filament preparation in the presence of Mg2+ ions, it was clearly observed that thinning of the filament (or splitting into subfilaments) occurred before shortening (or depolymerization).
动态光散射（DLS）方法为我们提供了有关溶液中颗粒的表观扩散系数Dapp以及静态散射强度Is的信息。对于长度为L、直径为d的长而细的棒状颗粒，Dapp对L和d的依赖性与Is的依赖性有很大不同。我们借助DLS研究了兔骨骼肌的合成肌球蛋白丝在pH 8.3和10℃的溶液中的情况。结果表明，Mg2+离子会导致丝变粗和变长，而ATP（和ADP）会导致丝变细和缩短（解聚）。当在Mg2+离子存在的情况下向丝制剂中加入ATP时，可以清楚地观察到，丝变细（或分裂成亚丝）发生在缩短（或解聚）之前。
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