Mount H T, Dreyfus C F, Black I B
Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, UMDNJ, Piscataway 08854.
Neuroreport. 1994 Dec 20;5(18):2497-500. doi: 10.1097/00001756-199412000-00023.
Nerve growth factor (NGF) has been shown to promote the survival of cultured cerebellar Purkinje cells, when tested in conjunction with metabotropic receptor activation. In the present study, we examined the effects of neurotrophin-3 (NT-3) and brain-derived neurotrophic factor (BDNF). Following in vitro exposure to NT-3 for 6 days, survival of the calbindin positive Purkinje cells was increased, relative to vehicle-treated controls. The total number of neurons was not affected. These observations suggest a specific action of NT-3 on the Purkinje cell population. Moreover, autoradiographic analysis revealed high affinity [125I]NT-3 binding sites, consistent with a direct action of this neurotrophin. Simultaneous treatment with a metabotropic receptor agonist did not alter the NT-3-elicited increase in cell number. This suggests that NT-3 regulates Purkinje cell survival by a mechanism distinct from the NGF response. When tested alone, or in the presence of metabotropic agonist, BDNF did not affect Purkinje cell number.
当与代谢型受体激活共同测试时，神经生长因子（NGF）已被证明可促进培养的小脑浦肯野细胞的存活。在本研究中，我们检测了神经营养因子-3（NT-3）和脑源性神经营养因子（BDNF）的作用。体外暴露于NT-3 6天后，相对于用赋形剂处理的对照组，钙结合蛋白阳性浦肯野细胞的存活率增加。神经元总数未受影响。这些观察结果表明NT-3对浦肯野细胞群体有特定作用。此外，放射自显影分析显示存在高亲和力的[125I]NT-3结合位点，这与这种神经营养因子的直接作用一致。用代谢型受体激动剂同时处理并未改变NT-3引起的细胞数量增加。这表明NT-3通过一种不同于NGF反应的机制调节浦肯野细胞的存活。单独测试或在存在代谢型激动剂的情况下测试时，BDNF均不影响浦肯野细胞数量。
