Ostrowska A
Polish Academy of Sciences, Institute of Animal Physiology and Nutrition, Jabłonna.
Biol Cell. 1994;81(3):223-6. doi: 10.1016/0248-4900(94)90004-3.
The activation of protein kinase A (PKA) by cAMP was estimated in supernatant fractions from the hypothalamus (Hyp) and anterior pituitary (AP) of the female rat during the oestrous cycle and in ovariectomized and ovariectomized, ovarian steroid hormone treated animals. In both structures, the largest activation of PKA was found in dioestrus-2, while the lowest one was in Hyp in dioestrus-1 and in AP in oestrus. Ovariectomy had no influence on cAMP-dependent activation of PKA from Hyp and AP. Treatment of ovariectomized rats with 17-beta-oestradiol (E2), progesterone (P) or both abolished the activation of PKA by cAMP from AP and had no effect on hypothalamic PKA. These results indicate that ovarian steroids act specifically on AP processes via cAMP dependent pathway and regulation of PKA activity.
在雌性大鼠发情周期以及卵巢切除和卵巢切除后用卵巢甾体激素处理的动物中，测定了下丘脑（Hyp）和垂体前叶（AP）上清液部分中cAMP对蛋白激酶A（PKA）的激活作用。在这两个结构中，PKA的最大激活作用出现在动情后期-2，而最低激活作用在动情期-1的下丘脑和发情期的垂体前叶中。卵巢切除对下丘脑和垂体前叶中cAMP依赖的PKA激活没有影响。用17-β-雌二醇（E2）、孕酮（P）或两者对卵巢切除的大鼠进行处理，消除了cAMP对垂体前叶PKA的激活作用，而对下丘脑PKA没有影响。这些结果表明，卵巢甾体激素通过cAMP依赖途径和PKA活性调节，特异性作用于垂体前叶的过程。
