Lees D, Pugnère D, Castel A, Raux O, Travo P
Centre de Recherches de Biochimie Macromoléculaire, UPR 9008 CNRS, U 249 INSERM, Université de Montpellier, France.
Cell Biol Toxicol. 1994 Dec;10(5-6):305-9. doi: 10.1007/BF00755775.
We present a simple, noninvasive, nondestructive all-purpose method for the quantitative evaluation of functional effects of xenobiotics with cultured cells and the work station for its routine, easy implementation. At present 1 to 150 cells growing in one to six dishes can be studied in parallel or otherwise at time intervals ranging from 10 s to 6 h or more, over periods of time ranging from a few tens of minutes to 3-4 days. Any aspect of cell physiological behavior can be studied (differentiation-dedifferentiation, migration, division, degeneration, death) without preliminary staining and/or fixation provided it results in optically visible changes.
我们提出了一种简单、无创、非破坏性的通用方法，用于通过培养细胞对异生物素的功能效应进行定量评估，并介绍了实现该方法常规、简便实施的工作站。目前，在一至六个培养皿中生长的1至150个细胞可同时进行研究，或以10秒至6小时或更长时间的时间间隔进行研究，研究时间段从几十分钟到3至4天不等。细胞生理行为的任何方面都可以在无需预先染色和/或固定的情况下进行研究（分化-去分化、迁移、分裂、退化、死亡），只要其会导致光学可见的变化。
