Xu-van Opstal W Y, Billardon C, Caillaud T, Carvajal-Gonzalez S, Colliot G, Bisconte J C, Rostene W
INSERM U.339, Hôpital St Antoine, Paris, France.
Cell Biol Toxicol. 1994 Dec;10(5-6):387-92. doi: 10.1007/BF00755787.
An automated system, TRAKCELL, was developed for the quantitation of cells in culture. It enabled cell counting, classification according to morphological cell characteristics and measurement of cell proliferation and differentiation. The system was tested on the toxic effect of ascorbic acid on rat brain catecholaminergic neurons in primary culture. In parallel, the effects of nerve growth factor, dexamethasone and forskolin on cell differentiation were studied using rat pheochromocytoma PC12 cells. The results show that the system permits rapid and reproducible measurements of cell density and of the morphological changes observed following various drug treatments.
开发了一种自动化系统TRAKCELL，用于定量培养中的细胞。它能够进行细胞计数，根据细胞形态特征进行分类，并测量细胞增殖和分化。该系统用于测试抗坏血酸对原代培养的大鼠脑儿茶酚胺能神经元的毒性作用。同时，使用大鼠嗜铬细胞瘤PC12细胞研究了神经生长因子、地塞米松和福斯高林对细胞分化的影响。结果表明，该系统能够快速且可重复地测量细胞密度以及各种药物处理后观察到的形态变化。
