Caillaud T, Opstal W Y, Scarceriaux V, Billardon C, Rostene W
Institut National de la Santé et de la Recherche Médicale, Hôpital Saint Antoine, Paris, France.
Mol Neurobiol. 1995 Apr-Jun;10(2-3):105-14. doi: 10.1007/BF02740670.
Several lines of anatomical, neurochemical, electrophysiological, and behavioral evidence suggest the existence of physiological interactions between neurotensin (NT) and the brain dopaminergic systems. Thus, NT has been shown to exert a neuroleptic-like action and could be implicated in the pathogenesis and treatment of schizophrenia. It is thus of particular importance to develop in vitro cell culture systems as models to study such interactions. Rat adrenal pheochromocytoma PC12 cells, which expressed high levels of tyrosine hydroxylase, were used in the present study. In contrast to rat brain cells in primary cultures, PC12 cells did not express functional NT receptors. However, they were able to express both NTmRNA and NT in response to NGF, forskolin, and dexamethasone. Those neurochemical modifications furthermore may be related to changes in the morphology of the PC12 cells in response to NGF, forskolin, and dexamethasone alone or in combination. These data suggest that PC12 cells may provide a useful model to study in vitro the regulation of both catecholamine and neurotensin phenotypes.
多条解剖学、神经化学、电生理学及行为学证据表明，神经降压素（NT）与脑多巴胺能系统之间存在生理相互作用。因此，NT已被证明具有类似抗精神病药物的作用，可能与精神分裂症的发病机制及治疗有关。因此，开发体外细胞培养系统作为研究此类相互作用的模型尤为重要。本研究使用了高表达酪氨酸羟化酶的大鼠肾上腺嗜铬细胞瘤PC12细胞。与原代培养的大鼠脑细胞不同，PC12细胞不表达功能性NT受体。然而，它们能够在神经生长因子（NGF）、福司可林和地塞米松的作用下表达NTmRNA和NT。此外，这些神经化学修饰可能与PC12细胞在单独或联合使用NGF、福司可林和地塞米松时形态的变化有关。这些数据表明，PC12细胞可能为体外研究儿茶酚胺和神经降压素表型的调节提供一个有用的模型。
