对一个新建立的甲状腺肿瘤细胞系进行荧光原位杂交（FISH）分析，结果显示存在t(1;19)(p35或p36.1;q13)，这表明断点位于19q13.3 - 13.4与19q13.4之间。

FISH analyses of a newly established thyroid tumor cell line showing a t(1;19)(p35 or p36.1;q13) reveal that the breakpoint lies between 19q13.3-13.4 and 19q13.4.

作者信息

Belge G, Garcia E, de Jong P, Bartnitzke S, Bullerdiek J

机构信息

Center for Human Genetics and Genetic Counselling, University of Bremen, Germany.

出版信息

Cytogenet Cell Genet. 1995;69(3-4):220-2. doi: 10.1159/000133968.

DOI:10.1159/000133968

PMID:7698017

Abstract

Translocations involving the long arm of human chromosome 19 are specific chromosomal alterations found in a subset of benign thyroid tumors. In the present communication we describe the establishment of an SV40-transformed cell line derived from a thyroid adenoma with a t(1;19)(p35 or p36.1;q13). Cytogenetic studies were carried out on this cell line, using a series of cosmids mapping along the long arm of chromosome 19. In situ hybridization with probes for RYR1, ATP1A3, BCKDHA, ERCC1, POLD1, and TRPT revealed that only TRPT mapped distal to the breakpoint of the translocation. The results indicated that the breakpoint is located between POLD1 and TRPT at the boundary between chromosome bands 19q13.3 and 19q13.4.

摘要

涉及人类19号染色体长臂的易位是在一部分良性甲状腺肿瘤中发现的特定染色体改变。在本报告中，我们描述了一个从患有t(1;19)(p35或p36.1;q13)的甲状腺腺瘤衍生而来的SV40转化细胞系的建立。使用一系列沿着19号染色体长臂定位的黏粒对该细胞系进行了细胞遗传学研究。用针对RYR1、ATP1A3、BCKDHA、ERCC1、POLD1和TRPT的探针进行原位杂交，结果显示只有TRPT定位于易位断点的远端。结果表明，断点位于POLD1和TRPT之间，在染色体带19q13.3和19q13.4的边界处。