小鼠D4多巴胺受体编码基因的分子克隆与特性分析

Molecular cloning and characterisation of the gene encoding the murine D4 dopamine receptor.

作者信息

Fishburn C S, Carmon S, Fuchs S

机构信息

Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.

出版信息

FEBS Lett. 1995 Mar 20;361(2-3):215-9. doi: 10.1016/0014-5793(95)00168-9.

DOI:10.1016/0014-5793(95)00168-9

PMID:7698326

Abstract

The murine D4 dopamine receptor was isolated from a murine genomic DNA library. The receptor's entire coding region was contained within a 6 kb EcoRI genomic fragment, indicating that the murine D4 receptor gene is significantly smaller than the corresponding D2 and D3 receptor genes, the coding regions of which each stretch over 30 kb. The murine D4 receptor gene has three introns and four exons, in common with the rat and human D4 receptor genes. RT-PCR on mRNA from different brain regions shows that the D4 receptor mRNA is expressed in various areas of the brain, with some differences from the rat and human receptor homologues.

摘要

小鼠D4多巴胺受体是从鼠基因组DNA文库中分离出来的。该受体的整个编码区包含在一个6 kb的EcoRI基因组片段中，这表明小鼠D4受体基因明显小于相应的D2和D3受体基因，后两者的编码区各自延伸超过30 kb。小鼠D4受体基因与大鼠和人类的D4受体基因一样，有三个内含子和四个外显子。对来自不同脑区的mRNA进行逆转录聚合酶链反应（RT-PCR）表明，D4受体mRNA在脑的各个区域均有表达，与大鼠和人类受体同源物存在一些差异。