人类和大鼠D2多巴胺受体基因的结构与表达

Structure and expression of human and rat D2 dopamine receptor genes.

作者信息

Araki K, Kuwano R, Morii K, Hayashi S, Minoshima S, Shimizu N, Katagiri T, Usui H, Kumanishi T, Takahashi Y

机构信息

Department of Neuropharmacology, Niigata University, Japan.

出版信息

Neurochem Int. 1992 Jul;21(1):91-8. doi: 10.1016/0197-0186(92)90071-x.

DOI:10.1016/0197-0186(92)90071-x

PMID:1363862

Abstract

D2 dopamine receptor may be related with the pathogenesis of Parkinson's disease and schizophrenia. Furthermore, the antipsychotic drugs have high affinity for D2 dopamine receptor. We carried out the cloning of the genomic DNA for human D2 dopamine receptor and clarified the structure of this gene. Our isolated gene spans about 15 kbp and consists of seven exons interrupted by six introns. However, putative first exon was not yet identified. Spot blot hybridization analysis of cell sorter fractionated human chromosomal DNA with D2 receptor genomic DNA revealed the localization of this gene in the chromosome 11 fraction. We analyzed human genomic DNA by Southern blot hybridization with D2 dopamine receptor genomic DNA as a probe, but so far we could not find RFLP. Northern blot analyses of brain RNA of several animals and rat brain RNA after various treatments were carried out. Developmental changes of D2 dopamine receptor mRNA were observed in the rat brains.

摘要

D2多巴胺受体可能与帕金森病和精神分裂症的发病机制有关。此外，抗精神病药物对D2多巴胺受体具有高亲和力。我们进行了人类D2多巴胺受体基因组DNA的克隆，并阐明了该基因的结构。我们分离出的基因跨度约为15千碱基对，由7个外显子和6个内含子间隔组成。然而，尚未鉴定出推定的第一个外显子。用D2受体基因组DNA对细胞分选的人类染色体DNA进行斑点杂交分析，揭示了该基因在11号染色体片段中的定位。我们以D2多巴胺受体基因组DNA为探针，通过Southern印迹杂交分析人类基因组DNA，但到目前为止我们尚未发现限制性片段长度多态性（RFLP）。我们对几种动物的脑RNA以及经过各种处理后的大鼠脑RNA进行了Northern印迹分析。在大鼠脑中观察到了D2多巴胺受体mRNA的发育变化。