Jeevaratnam P, Browett P J, Van de Water N S, Jass J R
Department of Molecular Medicine and Pathology, School of Medicine, University of Auckland, New Zealand.
Gut. 1995 Mar;36(3):382-4. doi: 10.1136/gut.36.3.382.
Restriction enzyme digests of genomic DNA show multiple alleles of common, intermediate, and rare frequencies at the minisatellite locus of Ha-ras. It has been suggested that a higher frequency of rare alleles is associated with the presence of colorectal and other types of cancer. This study investigated the distribution of Ha-ras alleles in 40 members of hereditary non-polyposis colorectal carcinoma (HNPCC) families and in 34 cancer free subjects (spouses). There was no difference in rare allele frequency between the cancer group and cancer free group (chi2 = 0.25, not significant).
基因组DNA的限制性酶切消化显示，在Ha-ras的小卫星位点存在常见、中等和罕见频率的多个等位基因。有人提出，罕见等位基因的较高频率与结直肠癌和其他类型癌症的存在有关。本研究调查了遗传性非息肉病性结直肠癌（HNPCC）家族的40名成员和34名无癌受试者（配偶）中Ha-ras等位基因的分布。癌症组和无癌组之间的罕见等位基因频率没有差异（χ2 = 0.25，无统计学意义）。
